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KMID : 0361020060490121159
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Korean Journal of Otolaryngology - Head and Neck Surgery
2006 Volume.49 No. 12 p.1159 ~ p.1164
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Minocycline Protects Vestibular Hair Cells from Neomycin-Induced Ototoxicity by Inhibiting Reactive Oxygen Species Production and Caspase-3 Activity
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Kim Young-Saeng
Ahn Jin-Chul
Baek In-Yeong
Rhee Chung-Ku
Jung Jae-Yun
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Abstract
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Background and Objectives : Aminoglycosides, a commonly used antibiotic agent, destroys the sensory hair cells in the cochlear and vestibular system leading to irreversible hearing loss and balance problem. Minocycline, a second-generation tetracycline antibiotic, has been known to possess anti-apoptotic properties in addition to its antimicrobial action. We hypothesized that minocycline would attenuate aminoglycosides induced vestibulotoxicity in rat utricles.
Subjects and Method : Utricular maculae prepared from postnatal day 3-4 rats were treated with neomycin alone or in combination with minocycline. For hair cell count, utricles were stained with phalloidin-FITC. Reactive oxygen species (ROS) was assessed using the fluorescent probe, hydrofluorescent diacetate acetyl ester (H2DCFDA). Caspase-3 activity was also examined using the fluorescent caspase-3 substrate.
Results : Neomycin induced dose-dependent loss of utricular hair cells. Minocycline reduced ROS production and caspase-3 activation in neomycin treated utricular hair cells.
Conclusion : Minocycline has protective effect in neomycin induced ototoxicity in rat utricle by inhibiting ROS production and caspase-3 activity.
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KEYWORD
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Minocycline, Caspase-3, Reactive Oxygen Species, Utricl
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